Regulatory

Part:BBa_K239003:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-22)


Modified HypB promoter, activated by Fnr during oxygen deficiency


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimisation of Fnr binding sites, modification of position 2 bp. Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first Fnr binding site and ends 35 bp after second Fnr binding site.


Source

Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.

References