Regulatory
Part:BBa_K239003:Design
Designed by: Axel Nystrom Group: iGEM09_UCL_London (2009-06-22)
Modified HypB promoter, activated by Fnr during oxygen deficiency
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimisation of Fnr binding sites, modification of position 2 bp. Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first Fnr binding site and ends 35 bp after second Fnr binding site.
Source
Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.